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lipe mouse pre designed sirna set a  (MedChemExpress)


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    MedChemExpress lipe mouse pre designed sirna set a
    Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting <t>siRNA</t> or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ <t>LIPE</t> ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001
    Lipe Mouse Pre Designed Sirna Set A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipe mouse pre designed sirna set a/product/MedChemExpress
    Average 94 stars, based on 3 article reviews
    lipe mouse pre designed sirna set a - by Bioz Stars, 2026-03
    94/100 stars

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    1) Product Images from "Hormone-sensitive lipase drives pro-resolving macrophage polarization and enhances efferocytosis"

    Article Title: Hormone-sensitive lipase drives pro-resolving macrophage polarization and enhances efferocytosis

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-025-02631-z

    Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting siRNA or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ LIPE ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting siRNA or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ LIPE ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Knockdown, Inhibition, Transfection, Control, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Derivative Assay, Whisker Assay



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    lipe mouse pre designed sirna set a  (MedChemExpress)
    94
    MedChemExpress lipe mouse pre designed sirna set a
    Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting <t>siRNA</t> or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ <t>LIPE</t> ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001
    Lipe Mouse Pre Designed Sirna Set A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipe mouse pre designed sirna set a/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    lipe mouse pre designed sirna set a - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

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    Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting siRNA or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ LIPE ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cell Communication and Signaling : CCS

    Article Title: Hormone-sensitive lipase drives pro-resolving macrophage polarization and enhances efferocytosis

    doi: 10.1186/s12964-025-02631-z

    Figure Lengend Snippet: Genetic knockdown of HSL replicates the effects of pharmacological inhibition, thereby confirming its essential role in sustaining the M2 phenotype. Human M0 macrophages were transfected with control non-targeting siRNA or siRNA targeting HSL. (A) Efferocytosis efficiency was measured 48 h post-transfection ( n = 6 independent donors). (B) Secreted levels of the pro-resolving mediator Annexin A1 and the pro-inflammatory cytokine TNFα in culture supernatants, measured by ELISA ( n = 9–10 independent donors). (C) Relative mRNA expression of M2-associated genes (PPARγ, CD163, CD36, CD200R1) analyzed by qRT-PCR. ( n = 5, independent donors). (D) Relative mRNA expression of M1-associated genes (TNFα, IL1β, HIF1α) and HSL/ LIPE ( n = 5, independent donors). (E) Efferocytosis was measured in murine M0 bone marrow-derived macrophages (BMDMs) following siRNA-mediated knockdown of HSL ( n = 6 biological replicates). For panels A, B, and E, a paired t-test was used. For panels C and D, a two-way ANOVA was used. Data in A, B, and E are presented as box-and-whisker plots. Panels C & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: For siRNA-mediated gene silencing, M0-human macrophages or BMDMs were transfected with 25 nM of Silencer pre-designed siRNA targeting human LIPE /HSL (143993 and 143994, Life Technologies), Lipe mouse pre-designed siRNA Set A (HY-RS18621, MCE), or a non-targeting control pool (Dharmacon, Lafayette, CO) using Lipofectamine LTX Transfection Reagent (Thermo Fisher Scientific).

    Techniques: Knockdown, Inhibition, Transfection, Control, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Derivative Assay, Whisker Assay